diff --git a/pc/jsep_transport_controller.cc b/pc/jsep_transport_controller.cc
index b7ab279862..9b14dc0342 100644
--- a/pc/jsep_transport_controller.cc
+++ b/pc/jsep_transport_controller.cc
@@ -92,8 +92,10 @@ JsepTransportController::JsepTransportController(
 JsepTransportController::~JsepTransportController() {
   // Channel destructors may try to send packets, so this needs to happen on
   // the network thread.
-  network_thread_->Invoke<void>(RTC_FROM_HERE,
-                                [this] { DestroyAllJsepTransports_n(); });
+  network_thread_->Invoke<void>(RTC_FROM_HERE, [this] {
+    RTC_DCHECK_RUN_ON(network_thread_);
+    DestroyAllJsepTransports_n();
+  });
 }
 
 RTCError JsepTransportController::SetLocalDescription(
@@ -104,6 +106,7 @@ RTCError JsepTransportController::SetLocalDescription(
         RTC_FROM_HERE, [=] { return SetLocalDescription(type, description); });
   }
 
+  RTC_DCHECK_RUN_ON(network_thread_);
   if (!initial_offerer_.has_value()) {
     initial_offerer_.emplace(type == SdpType::kOffer);
     if (*initial_offerer_) {
@@ -123,6 +126,7 @@ RTCError JsepTransportController::SetRemoteDescription(
         RTC_FROM_HERE, [=] { return SetRemoteDescription(type, description); });
   }
 
+  RTC_DCHECK_RUN_ON(network_thread_);
   return ApplyDescription_n(/*local=*/false, type, description);
 }
 
@@ -558,7 +562,6 @@ RTCError JsepTransportController::ApplyDescription_n(
     bool local,
     SdpType type,
     const cricket::SessionDescription* description) {
-  RTC_DCHECK_RUN_ON(network_thread_);
   RTC_DCHECK(description);
 
   if (local) {
@@ -955,7 +958,6 @@ RTCError JsepTransportController::MaybeCreateJsepTransport(
     bool local,
     const cricket::ContentInfo& content_info,
     const cricket::SessionDescription& description) {
-  RTC_DCHECK(network_thread_->IsCurrent());
   cricket::JsepTransport* transport = GetJsepTransportByName(content_info.name);
   if (transport) {
     return RTCError::OK();
diff --git a/pc/jsep_transport_controller.h b/pc/jsep_transport_controller.h
index f0adeedf26..64a599088e 100644
--- a/pc/jsep_transport_controller.h
+++ b/pc/jsep_transport_controller.h
@@ -229,7 +229,8 @@ class JsepTransportController : public sigslot::has_slots<> {
  private:
   RTCError ApplyDescription_n(bool local,
                               SdpType type,
-                              const cricket::SessionDescription* description);
+                              const cricket::SessionDescription* description)
+      RTC_RUN_ON(network_thread_);
   RTCError ValidateAndMaybeUpdateBundleGroup(
       bool local,
       SdpType type,
@@ -237,8 +238,10 @@ class JsepTransportController : public sigslot::has_slots<> {
   RTCError ValidateContent(const cricket::ContentInfo& content_info);
 
   void HandleRejectedContent(const cricket::ContentInfo& content_info,
-                             const cricket::SessionDescription* description);
-  bool HandleBundledContent(const cricket::ContentInfo& content_info);
+                             const cricket::SessionDescription* description)
+      RTC_RUN_ON(network_thread_);
+  bool HandleBundledContent(const cricket::ContentInfo& content_info)
+      RTC_RUN_ON(network_thread_);
 
   bool SetTransportForMid(const std::string& mid,
                           cricket::JsepTransport* jsep_transport);
@@ -295,12 +298,14 @@ class JsepTransportController : public sigslot::has_slots<> {
   RTCError MaybeCreateJsepTransport(
       bool local,
       const cricket::ContentInfo& content_info,
-      const cricket::SessionDescription& description);
+      const cricket::SessionDescription& description)
+      RTC_RUN_ON(network_thread_);
 
-  void MaybeDestroyJsepTransport(const std::string& mid);
-  void DestroyAllJsepTransports_n();
+  void MaybeDestroyJsepTransport(const std::string& mid)
+      RTC_RUN_ON(network_thread_);
+  void DestroyAllJsepTransports_n() RTC_RUN_ON(network_thread_);
 
-  void SetIceRole_n(cricket::IceRole ice_role);
+  void SetIceRole_n(cricket::IceRole ice_role) RTC_RUN_ON(network_thread_);
 
   cricket::IceRole DetermineIceRole(
       cricket::JsepTransport* jsep_transport,
@@ -334,24 +339,33 @@ class JsepTransportController : public sigslot::has_slots<> {
   std::vector<cricket::DtlsTransportInternal*> GetDtlsTransports();
 
   // Handlers for signals from Transport.
-  void OnTransportWritableState_n(rtc::PacketTransportInternal* transport);
-  void OnTransportReceivingState_n(rtc::PacketTransportInternal* transport);
-  void OnTransportGatheringState_n(cricket::IceTransportInternal* transport);
+  void OnTransportWritableState_n(rtc::PacketTransportInternal* transport)
+      RTC_RUN_ON(network_thread_);
+  void OnTransportReceivingState_n(rtc::PacketTransportInternal* transport)
+      RTC_RUN_ON(network_thread_);
+  void OnTransportGatheringState_n(cricket::IceTransportInternal* transport)
+      RTC_RUN_ON(network_thread_);
   void OnTransportCandidateGathered_n(cricket::IceTransportInternal* transport,
-                                      const cricket::Candidate& candidate);
-  void OnTransportCandidateError_n(
-      cricket::IceTransportInternal* transport,
-      const cricket::IceCandidateErrorEvent& event);
+                                      const cricket::Candidate& candidate)
+      RTC_RUN_ON(network_thread_);
+  void OnTransportCandidateError_n(cricket::IceTransportInternal* transport,
+                                   const cricket::IceCandidateErrorEvent& event)
+      RTC_RUN_ON(network_thread_);
   void OnTransportCandidatesRemoved_n(cricket::IceTransportInternal* transport,
-                                      const cricket::Candidates& candidates);
-  void OnTransportRoleConflict_n(cricket::IceTransportInternal* transport);
-  void OnTransportStateChanged_n(cricket::IceTransportInternal* transport);
+                                      const cricket::Candidates& candidates)
+      RTC_RUN_ON(network_thread_);
+  void OnTransportRoleConflict_n(cricket::IceTransportInternal* transport)
+      RTC_RUN_ON(network_thread_);
+  void OnTransportStateChanged_n(cricket::IceTransportInternal* transport)
+      RTC_RUN_ON(network_thread_);
   void OnTransportCandidatePairChanged_n(
-      const cricket::CandidatePairChangeEvent& event);
-  void UpdateAggregateStates_n();
+      const cricket::CandidatePairChangeEvent& event)
+      RTC_RUN_ON(network_thread_);
+  void UpdateAggregateStates_n() RTC_RUN_ON(network_thread_);
 
   void OnRtcpPacketReceived_n(rtc::CopyOnWriteBuffer* packet,
-                              int64_t packet_time_us);
+                              int64_t packet_time_us)
+      RTC_RUN_ON(network_thread_);
 
   void OnDtlsHandshakeError(rtc::SSLHandshakeError error);